STUDY OF THE EFFECT OF A GENE-MODIFIED PRODUCT (SOY) ON THE MICROFLORA OF THE COLON
Keywords:
GMO soy, white outbred rats, indigenous and facultative microflora, dysbiosisAbstract
Objective: GM-a comparative study of the degree of occurrence of representatives of the normal microflora of the colon of experimental animals indigen and facultative under the influence of soy.
Materiale and methods: To do this, 90 toothless male feathers were involved in the study, which were divided into 3 groups: group 1 - intact white toothless feathers (n = 30) in the standard vivarium diet, not containing soybeans without GM or GM; Group 2 - white rats (n = 30) comprising soy without GM in the standard vivarium diet; Group 3 - white rats (n = 30) coated with GM soy in the standard vivarium diet. Bacteriological researches of intestinal mass of proteinaceous invertebrates were conducted. In our research we studied at representatives of intestinal microflora of 9 levels of sight of microorganisms.
Results: The results showed that in both groups there were sharp differences in the degree of occurrence of representatives of this microflora. It was found that in white beardless rats, in whose diet GM soy was added, the microflora of the large intestine is indigenous (Bifidobacterium spp, Lactobacillus spp, lactosapasitive Escherichia coli) and facultative (Enterobacter spp, Proteus spp, Staphylococcus spp, Streptococcus spp, Candida spp, lactosanegative Escherichia coli ) the microflora of the main group is disturbed. It has been proven that a decrease in the level of indigenous microorganisms leads to an increase in the number of facultative microorganisms, the lactosanegative Escherichia coli germinates in large quantities, and an increase in the level of Candida spp is the main sign of an increase in dysbiosis in the main group, which leads to dysbiosis of the colon. It has been shown that the main factor causing this is GM soybeans..
Conclusions: Laboratory animals that consumed soybeans with GM were found to have a 2.33-7.52-fold decrease in the number of microorganisms planted inside the jugan compared to the control and comparative groups. In the controlling and comparative groups in one case it wasn't revealed in the main group in 86.7% of cases when in biological substance there was no grammatical Escherichia coli, it was specified that the microorganism changed the specifics under the influence of GM soy and gained pathogenic character. It has been proven that as a result of a sharp increase in the percentage of lactosanegative flour Escherichia coli, the level of occurrence of lactosapositive Escherichia coli has decreased steadily. Representatives of the facultative microflora differed convincingly from the control group in that the incidence rate of grammatical enterobacteria (Enterobacter spp, Proteus spp) did not differ from each other in the main and comparative groups. It was explained by inaccuracy of a shadow for an organism of white feathers, low resistance of strains of both microorganisms studied by factors of the external environment.
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