CLINICAL AND IMMUNOHISTOCHEMICAL CHANGES IN THE UPPER EYELID IN BLEPHAROCHALASIS IN WOMEN OF DIFFERENT AGE AND HORMONAL GROUPS
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blepharochalasis, upper eyelid, immunohistochemistry, ER-α, ER-β, estrogen receptors, menopause.##article.abstract##
Purpose of study: to evaluate the clinical and immunohistochemical characteristics of the upper eyelid skin in women with blepharochalasis from different age-hormonal groups based on the expression levels of estrogen receptors ER-α and ER-β. Materials and methods: after standart ophthalmological examination, 90 patients with blepharochalasis aged 24 to 67 years were divided into three groups according to age and hormonal status. Additionally, all patients underwent assessment of the condition of the upper eyelid skin, biopsy sampling, and immunohistochemical examination using monoclonal antibodies against estrogen receptors ER-α and ER-β. The level of receptor expression was evaluated using the H-score method. Results: the mean ER-α expression level in patients of reproductive age was 78.4±4.6, in the perimenopausal group 61.2±3.9, and in the postmenopausal group 44.7±3.2. A similar trend was observed for ER-β expression: 72.9±4.1, 58.6±3.4, and 39.5±2.8, respectively. Analysis of variance revealed statistically significant differences between the studied groups in the expression levels of ER-α (p=0.0003) and ER-β (p=0.0001). Conclusion: the obtained results indicate a decrease in estrogen receptor expression with increasing patient age and confirm the involvement of hormonal factors in the development of blepharochalasis.
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