MORPHOFUNCTIONAL CHANGES IN THE STOMACH WALL CAUSED BY ENERGY DRINKS
Keywords:
enzyme immunoassay, pepsinogen 1, pepsinogen 2, energy drink, blood serum, rat, stomachAbstract
Objective: Investigation of morphofunctional changes in the stomach wall in white rats aged 36 weeks exposed to energy drinks using laboratory research methods.
Methods: Healthy white male rats participated in the study. The experimental rats were divided into two groups. Morphofunctional changes in the stomach wall caused by the consumption of energy drinks in rat blood samples were evaluated using the enzyme immunoassay (ELISA). Blood samples taken for enzyme immunoassay were centrifuged and immunological tests were performed for pepsenogen 1 (pg1) and pepsenogen 2 (PG2), the cancer marker SA74-2 in serum samples.
Results: According to the analysis of the data obtained, it was found that the degree of severity of morphofunctional changes in the stomach wall in experimental animals of the main group depends precisely on the duration of action of the energy drink. A decrease in the ratio of PG1, PG2 in the blood and PG1/PG2 was observed to a lesser extent as a result of drinking an energy drink for 4 weeks. However, in the case of experimental rats who constantly (12 weeks) consumed an energy drink, it was found that the amount of PG1, PG2 decreased significantly. During the experiment, it was found that the specific serum antigen CA 74-2 increased by 23.5 times and a sharp decrease in the level of pepsinogen 1,2 and the ratio PG1/PG2, indicating the morphofunctional state of the stomach wall when taken for a long period of time, indicates damage to the glands of the stomach floor. The fact that the concentration of cancer markers CA 74-2 is sharply increased indicates the occurrence of metaplasia of glandular epithelial cells of the stomach wall.
Summary. Thus, the experiment showed that the level of changes in the morphofunctional state of the stomach wall in rats precisely depends on the duration of consumption of an energy drink. In 9-month-old rats who chronically consumed an energy drink, the level of PG1 increased by 79% and PG2 by 62%, the ratio of PG1/PG2 decreased by 46%, and the content of CA 74-2 increased by 23.5 times. The information provided indicates the appearance of symptoms of atrophy of the mucous membrane of the stomach wall
References
Белковец А.В. и соав. Oпыт неинвазивной диагностики атрофического гастрита в текущей клинической практике. //Бюллетень со рамн, том 33, № 4, 2013. С.71-76.
Белковец А.В. и соав. Неинвазивная диагностика фенотипа гастрита в клинической практике: анализ первой тысячи исследованний. //Экспериментальная и клиническая гастроэнтерология // выпуск 115 № 3 2015. C.26-30.
Зайцева А.А., Богданова Т.М. «Онкологические аспекты органов желудочно-кишечного тракта» ФГБОУ ВО «Саратовский государственный медицинский университет имени В. И. Разумовского Минздрава России, [Электронный ресурс] –URL: bogtanmih@mail.ru
Джураев М.Д., Худайбердиева М.Ш. Роль серологического опухоль-ассоциированного маркера СА-72-4 при ранней диагностике рецидива рака желудка. //Сибирский онкологический журнал. 2009. Приложение № 2. С. 63.
Решетников О. В., Курилович С. А., Рагино Ю. И., Молчанова А. Р., Сорокина Н. Н., Кротов С. А., Кротова В. А. Использование иммуноферментного анализа для выявления уровня пепсиногенов в крови. //Гастроэнтерология экспериментальная и клиническая №5/2013, C. 26-30.
Решетников О.В. и соав. Физиологическое и клиническое значение пепсиногенов желудка. // Клиническая медицина. № 3, 2014. C. 26-30.
Шалыгин Л.Д., Р.А. Еганян. Энергетические напитки — реальная опасность для здоровья детей, подростков, молодежи и взрослого населения. Часть 1. Состав энергетических напитков и влияние на организм их отдельных компонентов. //Профилактическая медицина, 1, 2016 10.17116. С. 56-63.
Mubarak R. Effect of Red Bull energy drink on rats’ submandibular salivary glands (light and electron microscopic study). //American Journal of Science, 2012, 8(1): P.366-372.
Raeesa A. Mohamed, Aly M. Ahmed, Tahani Ahmad Al-Matrafi, Ali H. AlRoalle, Musaad A. Alfayez, Deema M. Al-Okaiel, Ahmed F. El Fouhil, Muhammad Atteya, Energy drinks induce adverse histopathological changes in gastric and duodenal mucosae of rats //International Journal of Advanced and Applied Sciences, 5(2) 2018, Pages: 81-89.
Shaxanova Shaxnoza Shavkatovna , Raximov Nodir Maxammatkulovich , Koraboev Fayzullo Tojiboevich , Increase the effectiveness of treatment of multiple osteogenic metastases of kidney and prostate cancer, Journal of reproductive health and uro -nephrology research 2022, vol. 3, issue 2. pp
Samloff M.I. // Gastroenterol. 1982. V. 83. P. 204–209.
Sipponen P. // J. Clin. Gastroenterol. 2001. V. 32. P. 196–202.
Satoru T., Akira I., Hiroshi Y., Yuhkoh K. Serum Pepsinogen Levels in Normal and Experimental Peptic ulcer Rats Measured by Radioimmunoassay. // Chem. Pharm. Bull. 35 (4 )1515-1522(1987) / P.1015-1023.
Nargiza Yusupova, Oripov Firdavs, Eshqobilova Surayyo. Influence of energy drinks on individual systems of the human body //International Journal of Early Childhood Special Education (INT-JECSE) DOI:10.9756/INTJECSE/V14I5.899 ISSN: 1308-5581 Vol 14, Issue 05 2022/ P. 7176-7184
Nargiza Yusupova, Oripov Firdavs. Energy drinks. the composition of energy drinks and the effect on the body of their individual components /tjm - Tematics journal of Microbiology ISSN 2277-2952 Vol-6-Issue-1-2022/https://doi.org/10.5281/zenodo.6464588/P. 24-35/
O.A. Gelashvily. VARIANT OF PERIODIZATION OF BIOLOGICALLY SIMILAR STAGES OF HUMAN AND RAT’S ONTOGENESI// Саратовский наyчно-медицинский жyрнал № 4(22) 2008/ P.125-
Rizaev JA, Khazratov AI, Iordanishvili AK Morphofunctional characteristics of the mucous membrane of the masticatory apparatus in experimental carcinogenesis //Russian Journal of Dentistry. – 2021. – T. 25. – No. 3. – pp. 225-231.
