BIOAKTIV MTA-HP VA ENDOSEQUENCE BC SEMENTLARINING APLKAL NUQSONLARNI YOPISHDA QO‘LLANILISHI: YETILMAGAN DOIMIY TISHLARDA REGENERATIV POTENSIALNING TAQQOSLAMA MORFOMETRIK VA GISTOLOGIK TADQIQI
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bioaktiv sement; MTA-HP; EndoSequence BC; ProRoot MTA; apeksifikatsiya; KNKT morfometriyasi; Runx2; bolalar endodontiyasi##article.abstract##
Tadqiqot maqsadi — yetilmagan doimiy tishlarda apikal “biologik tiqin” hosil qilish jarayonida bioaktiv sementlar MTA-HP, EndoSequence BC va klassik ProRoot MTA ning regenerativ potensialini solishtirish. Klinik qismga 8–15 yoshdagi, bitta ildizli va keng apeksli tishlarga ega o‘ttiz nafar bemor kiritildi; har bir material bo‘yicha o‘nta holat. Dezinfeksiya 2,5 % NaOCl va 17 % EDTA bilan o‘tkazilgach, 3–4 mm apikal tiqin yaratildi, koronal qismi gutapercha bilan plombalandi; kuzatuv — 18 oy. KNKT morfometriyasi (Pixel Volume Histogram) yangi hosil bo‘lgan minerallashgan to‘qimaning o‘rtacha qalinligini aniqladi, Masson–Goldner bo‘yoqlari bilan gistologiya va Runx2 bo‘yicha immunogistokimyo minerallashuv darajasi hamda osteogen faollikni baholadi; nanoindentatsiya 28 sutkadan keyin sementlarning elastiklik modulini o‘lchadi. EndoSequence BC eng qalin apikal to‘siqni hosil qildi (3,2 ± 0,5 mm; ProRoot MTA ga nisbatan p < 0,05), MTA-HP esa minerallashuvning eng yuqori intensivligini (4,2 ± 0,3 ball) va Runx2 ekspressiyasining maksimal darajasini ta’minladi. Barcha materiallarda elastiklik moduli 19,5–21,0 GPa diapazonida bo‘lib, dentinga yaqin ko‘rsatkichlar qayd etildi, bu ularning mexanik mosligini tasdiqlaydi. Natijalar MTA-HP va EndoSequence BC apikal tiqin sifati bo‘yicha ProRoot MTA dan ustunligini ko‘rsatadi; MTA-HP zich biointegratsiya uchun optimal, EndoSequence BC esa keng apeks sharoitida hajmni tez to‘ldirish uchun ma’qul. Ish to‘qimalar regeneratsiyasini optimallashtirish va yuqori xavf guruhidagi bolalarda infeksiya qaytalanishini oldini olishda sementni to‘g‘ri tanlashning klinik ahamiyatini namoyon etadi.
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